Dr. Longmei Zhao’s Curriculum Vitae

POST – GRADUATE TRAINING

Fellowship    Cleveland Clinic Foundation, Cleveland, OH                                                          08/2020 – present Women’s Health (Breast/Gynecologic) Pathology

Resident       Cleveland Clinic Foundation, Cleveland, OH                                                        06/2016 – 06/2020 Anatomic / Clinical Pathology (AP/CP)

 

EDUCATION

 

Postdoc Stanford University, School of Medicine, Palo Alto,   CA 2007
 

Ph.D.

Stem Cell Biology

University of Illinois at Urbana-Champaign, Urbana,   IL

 

2005

 

Master

Molecular Biology

Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China

 

2002

 

M.D.

Cell Biology

Southeast University Medical College, Nanjing, China,

 

1998

  Clinical Medicine  
BOARD CERTIFICATION AND MEDICAL LICENSE

ECFMG  Certification

 

 

06/2015

Ohio Medical License 06/2016
California Medical License 01/2020
 

 

ACADEMIC AND RESEARCH EXPERIENCES

Research Projects during Residency and Clinical Fellowship Training

 

  1. Uterine stromal sarcomas: molecular and immunohistochemistrial
  2. Immunostaining patterns of p53 and p16 in high-grade serous carcinoma of the ovary with BRCA1/2
  3. Clinicopathologic features of endometrial carcinomas with heterogeneous loss of mismatch repair protein immunoexpression.
  4. Periductal stromal sarcoma of breast: pathohistological and immunohistochemical study of 6
  5. Dedifferentiated gastrointestinal stromal tumor: genetic and histopathological study of 26
  6. ABHD6 immunostain identifies a new molecular pathway implicated in human hepatocellular carcinoma pathogenesis.
  7. Discovery of novel mRNA/protein expression in bone and soft tissue
  8. Comparison of PDL1 antibody staining between surgical tissue and cytologic specimens for targeted immunotherapy in non-small cell lung
  9. Comparison of detection of molecular alternation between liquid-based cytology samples and cell block materials in non-small cell lung
  10. Comparison of monoclonal and polyclonal antibodies of surface light chain for determination of B-cell clonality by flow
  11. The correlation of cytology and microbiology in candida
  12. Determination of normal reference range of D-dimer in elderly
  13. Significance of low levels of nucleated red blood cells in peripheral

 

Selected Research Projects during Master, Ph.D, and Postdoctoral Fellowship Training

  1. Investigation of the role of cytokines in self-renewal and differentiation of adult mesenchymal stem
  2. Development of an effective method to isolate murine and human adult mesenchymal stem cells from fresh tissues.
  3. Study of the role of hair follicle stem cells in wound healing using a mouse
  4. Characterization of human epidermal and hair follicle stem cells by
  5. Development of a novel poloxamer-octapeptide hybrid hydrogel to support human mesenchymal stem cell proliferation and
  6. Discovery of two oligopeptides as novel potential shin-whitening
  7. Study of expression and function of SNARE associated proteins during fertilization using a knockout mouse model.
  8. Study of expression and cloning of the genes during spermatogenesis by differential display RT-PCR.

 

 

TEACHING AND EDUCATIONAL EXPERIENCE  
Lecturer, Kent State University, Cleveland, OH 05/2020
Presented lecture “Lipoproteins and Measurement” to the staffs in Department of Clinical Chemistry  
Lecturer, Cleveland Clinic Foundation, Cleveland, OH 08/2019
Presented lecture “Endometrial Stromal Tumor” to the residents and staffs in Pathology Department  
Lecturer, Kent State University, Cleveland, OH 07/2019
Presented lecture “Neutral Tube Defect and AFP” to staffs in Department of Clinical Chemistry  
Lecturer, Cleveland Clinic Foundation, Cleveland, OH 11/2018
Presented lecture “Gastrointestinal Stromal Tumor” to the residents and staffs in Pathology Department  
Lecturer, Cleveland Clinic Foundation, Cleveland, OH 07/2018

 

 

Presented lecture “Normal Histology of Breast” to the first year residents in Pathology Department  
Lecturer, Case Western Reserve University, Cleveland, OH 03/2018
Presented lecture “Cell-free DNA and NIPT” to graduate students in Department of Clinical Chemistry  
Lecturer, Case Western Reserve University, Cleveland, OH 01/2018
Presented lecture “Tumor Markers” to graduate students in Department of Clinical Chemistry  

Research Mentor, San Jose State University, San Jose, CA                                                        07/2009 – 12/2013 Supervised seven graduated interns (P. Abdollah, S. Do, B. Purandare, C. Nye, R. Gadkari, SG. Svendsen, T. Teklemariam) in stem cell internship laboratory-based learning program

Research Mentor, Stanford University, School of Medicine, Palo Alto, CA                                 05/2006 – 09/2006 Supervised undergraduate student David Chiang

 

PUBLICATIONS

Peer Reviewed Journal Articles/Reviews

  1. Zhao, L., Corless, C., Wang, , Wardelmann, E., Hornick, JK., Doyle, LA., Horvai, A., Agaimy, A., Yantiss, R., Rubin, B. Dedifferentiation in gastrointestinal stromal tumor occurs across all primary genetic subtypes and is characterized by secondary genetic mutations targeting cell cycle regulatory genes and the phosphatidyl inositol 3-kinase pathway. In Preparation
  2. Zhao, L., Cheng , Policarpio-Nicolas, ML. ESR1-NOCA2 Fusion Uterine Tumor Resembling Ovarian Sex Cord Tumor: Report of a Case Mimicking Low-grade Endometrial Stromal Sarcoma. In Preparation
  3. Zhao, L., Cheng , Policarpio-Nicolas, ML. ZC3H7B-BCOR Fusion Endometrial Stromal Sarcomas: Report of a Case Morphologic Mimicking Low-grade Endometrial Stromal Sarcomas. In Preparation
  4. Zhao, L., Andrea Dowson, A., Rowe, JJ. Periductal stromal sarcoma of breast: pathohistological and immunohistochemical study of 6 cases. In Preparation
  5. Teklemariam, , Zhao, L., Hantash, BM. Heterologous expression of mutated HLA-G1 reduces alloreactivity of human dermal fibroblasts. Regenerative Medicine. 2014, 9(6):775-84
  6. Zhao, L., Teklemariam, , Hantash, BM. Heterologous expression of mutated HLA-G decreases immunogenicity of human embryonic stem cells and their epidermal derivatives. Stem Cell Research. 2014, 13, 342-354
  7. Teklemariam, , Purandare, B., Zhao, L., Hantash, BM. Inhibition of DNA methylation enhances HLA-G expression in human bone marrow- and adipose-derived MSCs. Biochemical and Biophysical Research Communications. 2014, 452(3):753-9
  8. Gadkari, R., Zhao, L., Hantash, BM. Comparison of human embryonic stem cell-, adipose-, and bone marrow-derived mesenchymal stem cells. Regenerative Medicine. 2014, 9(4):453-65
  9. Zhao, L., Teklemariam, , Hantash, BM. Mutated HLA-G3 can reach to cell surface but does not inhibit cytotoxicity of natural killer cells. Cellar Immunology. 2014, 287(1):23-6
  10. Purandare, B., Teklemariam, , Zhao, L., Hantash, BM. Temporal HLA profiling and immunomodulatory effects of human adult bone marrow-derived and adipose-derived mesenchymal stem cells. Regenerative Medicine. 2014, (1):67-79
  11. Svendsen, , Hantash, BM., Zhao, L., Faber, C., Bzorek, M., Nissen, MH., Hviid, TM. The expression and functional activity of membrane-bound Human Leukocyte Antigen-G1 are influenced by the 3′ untranslated region. Human Immunology. 2013, 74(7):818-27
  12. Zhao, , Abdollah, P., Do, S., Nye, C., Hantash, BM. Novel negative selection marker CD54 enhances differentiation of human adipose-derived mesenchymal stem cells. Journal of Clinical & Cellular Immunology.

2013, S9: 005

  1. Zhao, L., Purandare, B., Zhang, J., Hantash, BM. β2-microglobulin-free HLA-G activates natural killer cells by increasing cytotoxicity and proinflammatory cytokine Human Immunology. 2013, 74(4):417-24
  2. Zhao, L., Teklemariam, , Hantash, BM. Characterization of monoclonal antibodies recognizing HLA-G: a comparative study. Tissue Antigen. 2012, 80(3):231-8
  3. Teklemariam, , Zhao, L., Hantash, BM. Full-length HLA-G1 and truncated HLA-G3 differentially increase HLA-E surface localization. Human Immunology. 2012, 73(9):898-905
  4. Zhao, L., Hantash, BM. Male androgenetic alopecia is due to hair follicle stem cell inactivation. Expert Review of Dermatology. 2011, 6(2):145-147
  5. Jiang, S., *Zhao, L., Teklemariam, , Hantash, BM. Small cutaneous wounds induce telogen to anagen transition of murine hair follicle stem cells. Journal of Dermatological Science. 2010, 60(3):143-150
  6. Wang , *Zhao L., Hantash BM. Support of human adipose-derived mesenchymal stem cell multipotency by a poloxamer-octapeptide hybrid hydrogel. Biomaterials. 2010, (19):5122-30
  7. Jiang S., *Zhao L., Purandare B., Hantash BM. Differential expression of stem cell markers in  human follicular bulge and interfollicular epidermal compartments. Histochemistry and Cell Biology. 2010, 133(4):455-65
  8. *Zhao, L., Jiang S., Hantash, BM. TGF-b-mediated up-regulation of TAZ induces osteogenic differentiation of bone marrow-derived mesenchymal stem cells. Tissue Engineering Part A. 2010, 16(2):725-33
  9. Ubeid, AA,*Zhao, L., Wang, , Hantash, BM. Short-sequence oligopeptides with inhibitory activity against mushroom and human tyrosinase. J Investigative Dermatology. 2009, 129(9):2242-9
  10. Hantash, BM., Zhao, L., Knowles, JA., Lorenz, Adult and fetal wound healing. Frontiers in Bioscience. 2008, 1(13):51-61
  11. Zhao, L., Reim, K., Brose, N., Miller, Complexin 1 knockout mouse sperm are subfertile due to defects in acrosome exocytosis. Reproduction. 2008, 136(3):323-334
  12. *Zhao, L., Burkin, HR., Li, , Shi, X., Reim, K., Brose, N., Miller, DJ. Complexin 1 is required for mammalian sperm acrosomal exocytosis. Developmental Biology. 2007, 309:236–244
  13. Zhao, L., Li, , Shi, X., Miller, DJ. Dynamin 2 associates with complexin and is found in the acrosomal region of mammalian sperm. Molecular Reproduction and Development. 2007, 74(6):750-757
  14. Burkin, HR., Zhao, L., Miller, Expression of CASK in the mammalian sperm acrosome and processing during epididymal maturation. Molecular Reproduction and Development. 2004, 68:500-506
  15. Shi X., Zhao, L., Pei, K., Lou, L., Jia, M. Screening the stage-specific expression gene from different germ cells of rat. J Reproductive Medicine. 2002, 11(z1):29-37
  16. Zhao, L., Jia, M. Gene expression during meiosis in spermatogenesis. Foreign Medical Sciences. 2002, 21(2):110-113

* These authors contributed equally

Peer Reviewed Book Chapter

  1. Zhao, L., Hantash, BM. Isolation and cultivation of human scalp interfollicular epidermal stem cells. Methods in Molecular Biology. (Turksen K, ed.), Springer: New 2013. 989:11-9
  2. Zhao, L., Hantash, BM. Human bugle stem cell: markers, selection, and perspective clinic application. Stem cells and cancer stem cells. (Hayat, MA. ed.), Springer: New 2012. 4:195-123
  3. Zhao, , Hantash, BM. TGF-β1 regulates differentiation of bone marrow mesenchymal stem cells. Vitamins and Hormones. (Litwack, G., ed.), Elsevier: San Diego. 2011, 87:127-142

Platform Presentations

  1. Zhao, L., Purandare, B., Zhang, J., Hantash, BM. β2-microglobulin-free HLA-G activates natural killer cells by increasing cytotoxicity and proinflammatory cytokine production. 2012, Sixth International Conference of HLA-G. Pairs,
  2. Zhao, L., Li, S., Lorenz, , Hantash, BM. Fibroblast differentiation of mouse bone marrow-derived mesenchymal stem cells. 2007, Fifty-second Annual Meeting of the Plastic Surgery Research Council, Palo Alto, CA.
  3. Zhao, L., Li, S., Shi, , Hantash, BM. FGF2 regulates self-renewal of mouse adipose- and bone marrow-derived mesenchymal stem cells. 2007, Fifty-second Annual Meeting of the Plastic Surgery Research Council, Palo Alto, CA.
  4. Zhao, L., Burkin, HR., Li, L., Shi, X., Reim, K., Brose, N., Miller, DJ. Complexin 1, not complexin 2, is required for mammalian sperm acrosomal exocytosis. 2005, Eighteenth Annual Cell & Molecular Biology and Molecular Biophysics Research Symposium, Urbana,
  5. Zhao, L., Burkin, HR., Li, L., Shi, X., Reim, K., Brose, N., Miller, DJ. Complexin 1 knockout mouse sperm are subfertile due to a defect in acrosomal exocytosis. 2005, Thirty-eighth Annual Meeting of Society for the Study of Reproduction, Quebec, QC,
  6. Zhao, L., Burkin, HR., Reim, K., Brose, N., Miller, DJ. Complexin 1 is required for mammalian sperm acrosomal exocytosis. 2004, Thirty-seventh Annual Meeting of Society for The Study of Reproduction, Vancouver, BC,

 

Poster Presentation

  1. Zhao, L., Dowson, A., Rowe, JJ. Periductal Stromal Sarcoma of Breast: Pathohistological and Immunohistochemical Study of 6 2020, College of American Pathologists (CAP) Annual Meeting.
  2. Zhao, L., Hou, , Cheng, Y., Robertson, S., Policarpio-Nicolas, M. CD56 and CD99 Immunoexpression in Uterine Stromal Sarcomas (Low-grade, High-grade and Undifferentiated). 2020, College of American Pathologists (CAP) Annual Meeting.
  3. Bruehl, FK., Cook, E., Sharma, A., Shetty, , Zhao, L., Shen, M., Robertson, J., Nakashima, MO. Significance of Low Levels of Nucleated Red Blood Cells in Peripheral Blood. 2020, Cleveland Clinic GME Patient Safety Day. Cleveland, OH.
  4. Zhao, L., Zhang, , Yang, B. p53, but not BRCA Mutational Status, Affects the p16 Immunostaining Patterns in High-Grade Serous Carcinoma of the Ovary. 2020, 109th Annual Meeting of United States and Canadian Academy of Pathology (USCAP), Los Angeles, CA.
  5. Hou, , Zhao, L., Cruise, M., Policarpio-Nicolas, ML. Clinicopathologic Features of Endometrial Carcinomas with Heterogeneous Loss of Mismatch Repair Protein Immunoexpression. Our Institutional Experience. 2020, 109th Annual Meeting of United States and Canadian Academy of Pathology (USCAP), Los Angeles, CA.
  6. Zhao, , Wang, W., Wardelmann, E., Horvai, A., Hornick, JK., Doyle, LA., Yantiss, R., Agaimy, A., Rubin, B. Dedifferentiated Gastrointestinal Stromal Tumor: Clinicopathologic Study of 22 Cases. 2019, 108th Annual Meeting of United States and Canadian Academy of Pathology (USCAP), National Harbor, MA.
  7. Terzioglu, M., Wang, , Habermehl, G., Korty, K., Shen, M., and Zhao, L., Marchant, K. Age adjusted D-dimer Cutoff Value. 2019, Cleveland Clinic GME Patient Safety Day. Cleveland, OH.
  8. Zhao, L.,  Reynolds,     Comparison  of  PDL1  antibody  staining  between  surgical  tissue  and    cytologic specimens for targeted immunotherapy in non-small cell lung carcinoma (NSCLC). 2018, Accepted  66th Annual Scientific Meeting of American Society of Cytology, Washington DC.
  1. Zhao, L., Gay, , His, ED. Comparison of Monoclonal and Polyclonal Antibodies of Surface Light Chain for determination of B-cell Clonality by Flow Cytometry. 2018, PLMI 9th Annual Education Conference, Cleveland, OH.
  2. Zhao, L., Doxtader, EE., Zhang, Reliable Detection of EGFR mutations by real-time PCR and ALK Gene Rearrangements by FISH in NSCLC Using Liquid-Based Cytology Samples: High Concordance with NGS Performed on Cell Block Material. 2017, 65th Annual Scientific Meeting of American Society of Cytology, Phoenix, AZ.
  3. Gadkari, R., Zhao, L., Hantash, BM. Comparison of human embryonic stem cell-, adipose-, and bone marrow-derived mesenchymal stem cells. 2013, California Institute for Regenerative Medicine Bridges Program Annual Trainee Meeting, San Francisco,
  4. Abdollah, , Zhao, L., Do, S., Nye, C., Hantash, BM. Novel negative selection marker CD54 enhances differentiation of human adipose-derived mesenchymal stem cells. 2012, California Institute for Regenerative Medicine Bridges Program Annual Trainee Meeting, San Francisco, CA.
  5. Abdollah, , Nye, C., Zhao, L., Hantash, BM. Novel selection marker enhances identification of human adipose-derived mesenchymal stem cells. 2012, Stem Cell Internship Laboratory-based Learning Program of San Jose State University Annual Meeting, San Jose, CA.
  6. Teklemariam, , Zhao, L., Hantash, BM. Heterologous expression of IT™ in human mesenchymal stem cells. 2010, California Institute for Regenerative Medicine Bridges Program Annual Trainee Meeting, San Francisco, CA.
  7. Do, S., Zhao, L., Hantash BM. Novel cell surface markers to distinguish human mesenchymal stem cells and fibroblasts. 2010, California Institute for Regenerative Medicine Bridges Program Annual Trainee Meeting, San Francisco, CA.
  8. Purandare, B., Do, S., Zhao, L., Hantash, BM. HLA profiling of human mesenchymal stem cells. 2010, California Institute for Regenerative Medicine Bridges Program Annual Trainee Meeting, San Francisco,
  9. Teklemariam, , Zhao, L., Hantash, BM. Cloning and stable expression of IT™ in human mesenchymal stem cells. 2010, Stem Cell Internship Laboratory-based Learning Program of San Jose State University Annual Meeting, San Jose, CA.
  10. Do, S., Zhao, L., Hantash, BM. A bioinformatics approach to identify novel selection markers to distinguish human mesenchymal stem cells and fibroblasts. 2010, Stem Cell Internship Laboratory-based Learning Program of San Jose State University Annual Meeting, San Jose,
  11. Purandare, B., Do, S., Zhao, L., Hantash, BM. Temporal profiling of HLA expression in human bone marrow- and adipose-derived MSCs. 2010, Stem Cell Internship Laboratory-based Learning Program of San Jose State University Annual Meeting, San Jose,
  12. Zhao, L., Lorenz, , Hantash, BM. TGF-β-mediated up-regulation of TAZ induces osteogenic differentiation of bone marrow-derived mesenchymal stem cells. 2007, Third Stem Cell Research & Therapeutics conference, San Diego, CA.
  13. Wang, , Zhao, L., Tafti, BA., Mahmood, M.B., Lorenz, HP., Hantash, BM. Characterization of a self-assembling oligopeptide for skin engineering. 2007, Third Stem Cell Research & Therapeutics conference, San Diego, CA.
  14. Zhao, L., Burkin, HR., Li, , Shi, X., Reim, K., Brose, N., Miller, DJ. Complexin 1 is necessary for acrosomal exocytosis. 2005, Fourteenth Gordon Research Conference in Fertilization and Activation of Development, Plymouth, NH.
  1. Zhao, L., Burkin, HR., Li, , Shi, X., Reim, K., Brose, N., Miller, DJ. Complexin 1 is found in mammalian sperm and required for acrosomal exocytosis. 2004, Forty-fourth Annual Meeting of The American Society for Cell Biology, Washington, DC.
  2. Zhao, L., Burkin, HR., Li, , Shi, X., Reim, K., Brose, N., Miller, DJ. Complexin 1 is found in mammalian sperm and required for acrosomal exocytosis. 2004, Seventeenth Annual Cell & Molecular Biology and Molecular Biophysics Research Symposium, Urbana, IL.
  3. Burkin, HR, Zhao, L., Reim, K., Brose, N., Miller, Complexin 1 is required for mammalian sperm acrosomal exocytosis. 2003, Sixteenth Annual Cell & Molecular Biology and Molecular Biophysics Research Symposium, Urbana, IL.

Patents

  1. Title: HLA-G-modified cells and methods. Inventors: Hantash, BM., Zhao, L.
  2. Title: TGF-beat-mediated osteogenic differentiation of mesenchymal stem cell. Inventors: Hantash, BM., Zhao, L. 2008.

Grant

Funder: California Institution for Regenerative Medicine Dates: 09/2010 – 08/2013

Title: Development of an immune tolerant hESC source for allogeneic cell therapy applications Role: Co-investigator (Leading Scientist)

 

SELECTED HONORS AND AWARDS

First Place of Cleveland Clinic GME Patient Safety Day Poster Competition                                                     2019

Cleveland Clinic Foundation Caregiver Celebrations Award                                                                       2017, 2018

Sigma XI (International Honor Society)    Full Membership                                                                                 2008

Burroughs  Welcome  Trainee  Research Awards                                                                                                      2005

Larry  Ewing  Memorial  Trainee Travel Fund                                                                                                            2005

Travel Award  from Reproductive Program of University  of Illinois                                                         2004, 2005

David H. and Norraine A. Baker Graduate Fellowship in Animal Sciences at University of Illinois 2004 – 2005

Research Award from British Columbia Research Institute for Children’s &   Women’s Health                     2004

The Third-Prize Scholarship of Southeast University  Medical College                                                    1996 – 1997

 

LEADERSHIP & PROFESSIONAL AFFILIATIONS

CAP  Resident  Forum  Delegate                                                                                                      2018  –  2020

CCF  Pathology  Program  Evaluation  Committee                                                                      2018

American  Society  of  Cytopathology, Activate member                                                            2017  – present

American  Board  of  Pathology, Activate member                                                                       2016  – present

The College of American Pathologist,   Activate member,                                                           2016  – present

United States and Canadian Academy of Pathology,    Activate member,                                2016 – present

American  Society for  Clinical  Pathology, Activate member,                                                    2016  – present

The Association  of  Chinese American Physician,  Activate member                                       2016  – present

International  Society  for  Stem  Cell  Research, Activate  member                                          2007

 

 

American  Society  of  Cell  Biology,  Activate  member 2005, 2007
Society  for  the  Study  of  Reproduction,  Full member   2007
Society  for  the  Study  of  Reproduction,  Trainee  membership 2004, 2005
LANGUAGE FLUENCY

Chinese (Native) English (Advanced)

HOBBIES & INTERESTS

Visual art, hiking, jogging.

   

 

< Back to Pathologists